I have several related questions to that issue.
Let's say I have an album with some number of tracks in it that I normalized using "Analyze Volume" manually, at a dB offset setting of my choice.
Now, what happens when I import another track of that album from some source--for example, I record it from a streaming source?
Does MB run "Analyze Volume" on the import using a default 0 dB offset? Edit: Yes. I just tried it.
Does MB run it as is, with whatever offset is currently set (the dB offset setting appears to be sticky in that it doesn't revert to 0 after use or restart). Edit: No. It runs a vanilla analysis at 0dB offset.
Finally, if a track is added to an album that had previously been normalized at some setting, would it be preferable then to strip the gain tags and then redo the entire album so that album-relative volume is then established for all tracks?
Answer: Probably yes, if you have normalized your library to play at similar volume on an album basis.
I looked at the track gains that came in on seven tracks that I recorded from a streaming classical station, and compared those tracks to others in the album that I had imported previously and normalized at my preferred setting of -3.5dB. The new files looked suspiciously high in value, so I cleared the entire album and ran it again at my setting. The numerical difference was exactly 3.5dB on the high side.
So I conclude that MB will normalize new files added at a default 0 dB offset, not at whatever you might have last used when Analyzing Volume manually.