That probably brings your quest in this thread to an end then, since your issue doesn't seem to be related to MusicBee.
You could do a test with a foobar2000 portable installation (fb is pretty much the golden standard when it comes to playing audio on a Windows PC), but if Windows' own testing is failing for you already, foobar will probably too.
Other suggestions: look for support at the manufacturer of your dac, and search the web for other people having similar issues with it.
And maybe name the brand/model here, so others googling the same issue and landing here are informed?
And if you found a cure that might benefit other MusicBee users, be sure to report back?
Good luck!
p.s.
Repeating myself perhaps annoyingly: just re-encode everything to 16/44, delete the hi-res files and be done with it.
If you are unsure about this, see if you can setup a truly objective blind test first.
And be sure to remove yourself from the equation and ask a friend to assist.
I might be wrong, but I can't imagine you'll be able to tell what's what.
Well, maybe if the 16/44 files play 1dB louder, then you'll certainly prefer those. ;-)
If you decide to do this, starting a new topic in 'Beyond MusicBee' might be interesting...