I'm interest in this topic too. I usually have autoplaylists with multiple rules, random X songs, and usually one of those rules is to exclude the songs into a particular NOTautoplaylist.
When I transfer a chunk of music into my smartphone (syncing the autoplaylist), I usually then select all the songs in the autoplaylist, and drag them into the NOTautoplaylist, so the next time I'm sure that I can't listen the same songs again, until I have all my the songs in the NOTautoplaylist. At that point, I empty the NOTautoplaylist, and start anew.
But, sometime when I select the autoplaylist, it refreshes, so it select other random songs, so I can't drag them into the NOTautoplaylist. So I'm interested in the switching on/off the autoplaylist refresh, if it's possible.